Optimization of Triamcinolone Acetonide-loaded Solid Lipid Nanoparticles Formula by Central Composite Design and Response Surface Methodology

OBJECTIVE To optimize the formula of triamcinolone acetonide-loaded solid lipid nanoparticles(TACA-SLN). METHODS Central composite design and response surface methodology was applied to screen TACA-SLN prescription. The influence factors such as F68 concentration, the ratio of glyceryl monostearate to lecithin, the ratio of drug to lipids, the ratio of water phase to organic phase were investigated using entrapment efficiency as evaluation parameter. TACA-SLN was prepared by the method of emulsion evaporation at a high temperature and solidification at a low temperature. RESULTS The optimum prescription was: F68 1.89%, the ratio of glyceryl monostearate to lecithin was 1.95, the ratio of drug to lipids was 0.15, the ratio of water phase to organic phase was 5.68. The bias was 0.07%. CONCLUSION Cental composite design and response surface metogology was successfully used to optimize the formulation of TACA-SLN.