Determination of Anthraquinone in Hongquhuangtong Pill by HPLC

OBJECTIVE To establish a method for determining the content of anthraquinone in Hongquhuangtong pill by HPLC. METHODS Waters sunfire C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was consisted of methanol, acetonitrile and 0.1% phosphoric acid (25∶40∶35). The flow rate was 1.0 mL·min^-1. The column temperature was set at 35 ℃. The detector wavelength was 254 nm. RESULTS Emodin, chrysophanol, and physcion showed a good linear relationship with range in 11.50-115.04 ng, 12.24-122.40 ng, and 4.87-48.71 ng, respectively; and the recovery were 100.6%, 101.4%, and 97.7%. CONCLUSION The method is simple, rapid, accurate and reliable. And it can be applied to control the quality of anthraquinone from Hongquhuangtong pill.