Content Determination of Zafirlukast in Zafirlukast Tablets by HPLC

OBJECTIVE To establish a method for the content determination of zafirlukast in Zafirlukast Tablets by HPLC. METHODS Zorbax Eclipse Plus C₁₈(250 mm×4.6 mm，5 μm) column was used with 0.05 mol·L^-1 potassium dihydrogen phosphate (adjust pH to 3.0 with phosphoric acid)-acetonitrile (25∶75) as mobile phase. The flow rate was 1.0 mL·min^-1 and the detection wavelength was 242 nm. RESULTS The linear rang of zafirlukast was 20-70 μg·mL^-1(r=0.999 7, n=6), the average recovery was 100.1%, RSD was 0.61%. CONCLUSION The method is simple, reliable, accurate and can be used in the quality control of zafirlukast in Zafirlukast Tablets.