Simultaneous Determination of Eight Active Components in Chuanxiong Rhizoma from Different Areas by UPLC

OBJECTIVE To develop a UPLC method for determination of caffeic acid, vanillin, ferulic acid, senkyunolide I, senkyunolide H, butylphthalide, ligustilide and butylidenephthalide in Chuanxiong Rhizoma from different areas. METHODS The analysis was carried out on an HSS T3 (2.1 mm×100 mm, 1.8 μm) column. The mobile phase was composed of 0.03% phosphoric acid aqueous and methanol with gradient elution. The detection wavelengths were set at three different wavelengths according to different components. RESULTS The standard curves of eight active components showed a good linearity in 0.082 8-3.312 μg·mL^-1, 0.047 85-1.914 μg·mL^-1, 4.420-176.8 μg·mL^-1, 2.035-81.4 μg·mL^-1, 0.470 4-18.82 μg·mL^-1, 1.044-41.75 μg·mL^-1, 13.55-542.0 μg·mL^-1, 1.785-71.40 μg·mL^-1. The average recoveries were 101.0%, 99.1%, 98.9%, 103.6%, 96.3%, 102.8%, 98.1%, 98.0%, respectively. CONCLUSION This method is simple, quick, reproducible and can be used to control the quality of Chuanxiong Rhizoma and its preparations.