Simultaneous Determination of Salidroside and Chlorogenic Acid in Sargentodoxa Cueata by HPLC

OBJECTIVE To establish a wavelength conversion method for determination of salidroside and chlorogenic acid in Sargentodoxa cueata. METHODS The separation was performed on a Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm). Acetonitril-0.1% H₃PO₄ was used as the mobile phase with gradient elution. The flow rate was 1 mL·min^-1. The detection wavelength was 220 nm and 325 nm. RESULTS The linear range was 0.061 7-1.234 μg(r=0.999 7) and 0.030 2-0.605 μg (r=0.999 9), respectively. The average recoveries(n=6) of salidroside and chlorogenic acid were 97.8% and 98.1%, respectively. CONCLUSION This method is simple, sensitive and it may be used for the quality control of Sargentodoxa cuneata.