Simultaneous Determination of Allantoin and Nicotinamide in Cream by HPLC

OBJECTIVE To establish an HPLC method for determination of allantoin and nicotinamide in cream. METHODS Zorbax SB-C₁₈ column(250 mm×4.6 mm, 5 μm) was used as stanationly phase with methanol-0.02 mol·L^-1 potassium dihydrogen phosphate(25∶75) as mobile phase. The flow rate was 0.5 mL·min^-1, the column temperature was 30 ℃, and the detection wavelength was set at 217 nm. RESULTS The method had good linear relationship within the range of 4.2-21.0 μg·L^-1(r=0.999 9) for allantoin and 8.0-40.0 μg·L^-1(r=0.999 7) for nicotinamide, respectively. The results of the average recovery were 99.4%(RSD=0.27%) and 99.7%(RSD=0.25%). CONCLUSION The method is simple, rapid and accurate, and it can be used for the quality control.