Rapid Determination of Magnolol and Honokiol in Xiangshayangwei Pill by Near-infrared Spectroscopy

OBJECTIVE To fast determining the content of magnolol and honokiol in Xiangshayangwei pill by Near-infrared spectrum technology. METHODS The near infrared spectra of samples were collected, while take the HPLC value as a reference. The partial least squares (PLS) was used to establish the quantitative model of magnolol and honokiol. RESULTS The correlation coefficient of calibration (R²), the root-mean-square error of calibration(RMSEC) and the root-mean-square error of cross-validation(RMSECV) of the calibration model were 0.994 9, 0.011 1 and 0.020 5, respectively. The correlation coefficients of prediction(r²) of the validation and the root-mean-square error of prediction(RMSEP) were 0.988 3 and 0.016 7, respectively. CONCLUSION The method is simple and the result is accurate. It could be applied to determining the content of Xiangshayangwei pill in bulk and fast. It could save costs and improve efficiency.