Preparation and Characterization of DiI Loaded PLGA Nanoparticles

OBJECTIVE To prepare DiI loaded nanoparticles using PLGA and MePEG-PLGA as carriers. METHODS Spontaneous emulsion solvent diffusion method(SESD) was adopt to prepare nanoparticles. Ultrafiltration-UV-visible spectrophotometry was used for the determination of DiI-NP' encapsulation efficiency. RESULTS When DiI’s dosage was 0.5 mg, PLGA’s or MePEG-PLGA’s dosage was 50 mg, PVA’s content was 0.5%, the suitable nanoparticles were obtained. DiI-PLGA-NP’ average diameter was (280.7±3.6)nm, DiI-PLGA-NP’ Zeta potential was (-2.98±0.47)mV, DiI-PLGA-NP’ encapsulation efficiency was 88.0%; DiI-MePEG-PLGA-NP’ average diameter was (157.2±3.2)nm, DiI-MePEG-PLGA-NP’ Zeta potential was (-4.90±0.54)mV, DiI-MePEG-PLGA-NP’ encapsulation efficiency was 87.1%. CONCLUSION As a liposoluble long-term tracer, DiI-NP can lay the foundation for subsequent in vitro tests.