Effect of FGF21 in TGF-β Signaling Pathway of HepG2 Cells

OBJECTIVE To study the effects of FGF21 on relative proteins and mRNA expression involved in TGF-b/Smads signaling pathway. METHODS Detect the protein expressions of TGF-β1, TGF-β RⅡ, Smad 2,3,4,7 of HepG2 cells with Western blot and detect the mRNA expression of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 of HepG2 cells with Q-PCR. RESULTS The expressions of the protein and mRNA of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 varied with the different concentrations of FGF21(P<0.05). CONCLUSION FGF21 might inhibit tumor by promoting TGF-β signaling pathway in the expression of signaling molecules or receptors, or by decreasing the blocking molecules of this signaling pathway.