HPLC Fingerprint Study of Hawthorn Leaf from Chengde

OBJECTIVE To establish a sensitive and specific HPLC method and a standard fingerprint for quality control of hawthorn leaf from Chengde. METHODS With an Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm), gradient elution was performed by mobile phase of 0.5% formic acid-acetonitrile-methanol-tetrahydrofuran. While 0-15 min, detection wavelength was set at 260 nm, reference wavelength 360 nm; 15-65 min, detection wavelength 370 nm, reference wavelength 430 nm. The flow rate was 1.0 mL·min^-1, the column temperature was 30 ℃, injection volume was 10 μL. Fifteen batches of hawthorn leaf were analyzed with the developed HPLC fingerprint. The data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM(Version 2004A). RESULTS Twelve common peaks were found from 15 batches of hawthorn leaf HPLC fingerprint, of which seven peaks were confirmed, their similarities was greater than 0.9. CONCLUSION This method is accurate, reproducible and provides a scientific basis for quality control of hawthorn leaf.