Determination of Agomelatine in Tablets by HPLC

OBJECTIVE To establish a method for the content determination of Agomelatine tablets by HPLC. METHODS The HPLC method was performed on Phenomenex Luna-C₁₈ column (250 mm×4.6 mm, 5 μm) with mobile phase of methanol-0.05 mol·L^-1 sodium dihydrogen phosphate (40∶60). Flow rate was 1.0 mL·min^-1. Detective wavelength was set at 230 nm and the column temperature was set at 25 ℃. RESULTS The calibration curve of Agomelatine was linear Y=1.979×10⁵X-1.258×10⁴ (r=0.999 9, n=5) in the range of 1.07-10.7 μg·mL-1. The average recovery of Agomelatine was 98.73% (n=9) with RSD of 1.28%. CONCLUSION The method is simple, accurate, specific and it can be used for the determination of the content of Agomelatine tablets.