Determination of Eupalinolide A, B and Hyperin in Eupatorium Lindleyanum DC by HPLC

OBJECTIVE To establish a method for the determination of the content of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC from 6 different areas by HPLC. METHODS For eupalinolide A, B, Agilent Extend-C₁₈ column (4.6 mm×150 mm, 5 μm) was used under the mobile phase of acetonitrile-water(27∶73) at the flow rate of 1.0 mL·min^-1. The detection wavelength was 220 nm and the column temperature was maintained at 30 ℃. For hyperin, SB-C₁₈ column was used under the mobile phase of acetonitrile-1% Acetic acid(12∶88) at a flow of 1.0 mL·min^-1. The detection wavelength was 255 nm and the column temperature was maintained at 30 ℃. RESULTS The standard curve of eupalinolide A was linear in the range of 0.02-1.88 μg and eupalinolide B was linear in the range of 0.44-4.44 μg. The standard curve of hyperin was linear in the range of 0.101-1.414 μg. CONCLUSION This method was simple, accurate and reproducible for the determination of eupalinolide A, B and hyperin in Eupatorium lindleyanum DC.