Content Determination of Chlorogenic Acid and 4,5-O-Dicaffeoylquinic Acid in Chrysanthemi Indici Flos from Different Areas

OBJECTIVE To determine the content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos from different areas. METHODS HPLC was used. The chromatographic separation was performed on a Diamonsil C₁₈(4.6 mm × 250 mm, 5 μm) column with gradient elute. The detection wavelength was 327 nm and column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of chlorogenic acid and 4,5-O-dicaffeoylquinic acid was achieved when the amount was within 0.011-0.22 μg, 0.0175-0.35 μg; the recoveries were 99.1% and 98.8%; RSD were 1.93% and 1.54%(n=9). CONCLUSION The method is accurate, reproducible and suitable to determine chlorogenic acid and 4,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos.