Determination of Resveratrol in Cissus Hexangularis by HPLC

OBJECTIVE To develop an HPLC method for detecting resveratrol in Cissus hexangularis. METHODS An XB-C18 column(4.6 mm×250 mm, 5 mm) was used. The mobile phase consisted of acetonitrile-water(25∶75). The detection wavelength was 306 nm and the column temperature was room temperature. RESULTS Resveratrol showed a good linear relationship in the range of 2.50-40.0 μg·mL-1, r=0.999 9. The recovery and RSD were 97.5% and 0.98%(n=6), respectively. CONCLUSION This method is simple, accurate and repeatable to determine the content of resveratrol in Cissus hexangularis.