Simultaneous Determination of Three Components in Compound Tinidazole Carrier Suppositories by HPLC

OBJECTIVE To establish an HPLC method to simultaneous determine three components in compound tinidazole carrier suppositories. METHODS A Diamonsil-C₁₈(200 mm×4.6 mm, 5 μm) column was used; the mobile phase was the mixture of methanol-water-triethylamine(60∶40∶0.5)(adjusted by phosphoric acid to pH 4.0), the flow rate was 1.0 mL·min^-1, the UV detector was operated at 260 nm, injection volume was 20 μL; column temperature was 25 ℃. RESULTS The linear ranges of three components in compound tinidazole carrier suppositories tinidazole, clotrimazole, chlorhexidine acetate were 40–600, 32–480, 1.6–24 μg·mL^-1(r=0.999 9). The average recoveries respectively were 99.81%, 100.2%, 100.3%, and RSDs respectively were 0.37%, 0.2% and 0.4%(n=9). CONCLUSION This method can simultaneously determine three components. It is easy and fast with accurate and reliable results.