Study on Allergic Reaction Induced by Chuankezhi Injection

OBJECTIVE To establish a method of rapid test for allergic reaction induced by Chuankezhi injection(CI). METHODS The guinea pigs were sensitized continuously by intravenous injection of drugs once a day for three days, IL-4 and total IgE of serum were detected with ELISA method on the 8th day. The results were compared with traditional method of sensitization by intraperitoneal injection. RESULTS The allergic reaction didn’t occur on the 8th day after guinea pigs were sensitized by intravenous injection of three batches of CI, but IL-4 and total IgE of serum increased obviously and allergic reaction occurred from the 8th day after sensitized by CI added with 1.5 g·L^-1 chlorogenic acid, the onset time of allergy was reduced by 1-2 weeks as compared with traditional method, and the results were in accordance with each other. CONCLUSION It provides a new way of rapid test for CI induced allergic reaction.