Content Determination of Quercetin in Brassica Rapa L. by HPLC

OBJECTIVE To establish the content determination method of quercetin in Brassica rapa L., and so as to provide reference for its quality control. METHODS The content of quercetin in Brassica rapa L. was determined by HPLC. The chromatographic conditions were as follows: Kromasil C₁₈(4.6 mm×250 mm, 5 μm), mobile phase was methanol-0.1% phosphoric acid(50∶50), flow rate 1.0 mL·min^-1, column temperature at 30 ℃, detection wavelength 360 nm. RESULTS Querectin showed a good linear relationship in the range of 2.525 6-15.153 6 μg·mL^-1, the average recovery was 101.4% and RSD was 1.1%. CONCLUSION The established method is simple and rapid with good reproducibility, and can be used for the quality control of Brassica rapa L.