Study on the Synthesis Processing and Content Determination of Corn Silk Polysaccharide Selenide

OBJECTIVE Using the polysaccharide from corn silk as the raw material, to study the selenizing process with sodium selenite. METHODS Selenizing conditions were established by single factor and orthogonal design; determining content of polysaccharide selenide by Se(Ⅳ)-KSCN-MV extraction spectrophotometry; characterizing the structure of polysaccharide selenide by IR. RESULTS The optimal conditions were: 70 ℃ reaction temperature, 8 hours reaction time, mass ratio of corn silk polysaccharide to sodium selenite 1∶1.2, 0.3% HNO3 of volume fraction. The average content of selenium in corn silk polysaccharide was over 3.17 mg·g^-1 and average yield was 35.72%. IR results showed that corn silk polysaccharide contained Se=O and Se-C. CONCLUSION Corn silk polysaccharide selenide is successfully synthesized and the study provides the foundation for further study and exploiting for corn silk.