Protective Effects of Supercritical CO₂ Extracts of Armeniacae Semen Amarum on Human Fibroblast Damaged by Ultraviolet A

OBJECTIVE To observe the protective of supercritical CO₂ extracts of Armeniacae Semen Amarum on human fibroblasts iniured by irradiation of ultraviolet A and explore its possible mechanism. METHODS The model of photoaging fibroblasts was established by irradiating of ultraviolet light(20 J·cm^-2). The different concentration of supercritical CO₂ extracts of Armeniacae Semen Amarum were used for photoaging cell. The cell viability, SOD activity, GSH-Px activity, MDA content were assayed by MTT, hydroxylamine, colorimetric and TBA methods, respectively. The secretion levels of TNF-α and IL-1β of cultured ESF-1 cells were detected by ELISA. RESULTS After the UVA irradiation, the cell viability, the activities of SOD and GSH-Px were decreased, the content of MDA, TNF-α, and IL-1β were increased(P<0.01). The above changes were inhibited(P<0.05 or P<0.01) by 40 μg·mL^-1 and 80 μg·mL^-1 supercritical CO₂ extracts of Armeniacae Semen Amarum. CONCLUSION The supercritical CO₂ extracts of Armeniacae Semen Amarum might inhibit the proliferation decreasing which induced by UVA. And its mechanism may have relationship with the ability of strengthening antioxidation, decreasing oxygen radicals and decreasing the secretions of proinflammatory cytokines.