Study on Dynamic Changes of Ferulic Acid in Ferula Sinkiangensis in Different Phenological Period

OBJECTIVE To establish a method for determination of the content of ferulic acid in Ferula sinkiangensis, and to determine the content of ferulic acid in Ferula sinkiangensis in different phenological period. METHODS The content of ferulic acid was determined by HPLC. The determination was performed on Symmetry Shield RP₁₈(3.9 mm×150 mm, 5 μm) column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solvent(20∶80). The UV wavelength was set at 323 nm. The flow rate was 1 mL·min^-1 and column temperature was 30 ℃. RESULTS The linear range of ferulic acid in Ferula sinkiangensis were 1.92-11.50 μg·mL^-1(r=0.999 8) with a mean recovery rate of 99.6％ and RSD was 1.50％(n=9). The content of ferulic acid in Ferula sinkiangensis of different years was within the range of 0.063-0.236 mg·g^-1. Especially the content of ferulic acid in Ferula sinkiangensis growed for two years was the highest. CONCLUSION The method is specific, reliable and accurate. It can be used for the quality control of Ferula sinkiangesis effectively.