Determination of Monosaccharide and Disaccharide in Tanshinon Extract by HPLC-ELSD

OBJECTIVE An HPLC-ELSD method was established for the simultaneous quantitative analysis of monosaccharide, disaccharide from Tanshinon extract. METHODS The chromatographic separation was achieved on PrevailTM Carbohydrate ES column using a mobile phase composed of a mixture of acetonitrile and water by gradient elution at a flow rate of 0.8 mL·min^-1. The ELSD drift tube temperature was set at 60 ℃ and the carrying gas was air. RESULTS Monosaccharides and disaccharides from Tanshinon extract could be well separated, and the linear ranges of fructose, glucose and sucrose were 0.246-1.230 mg, 0.236-1.182 mg and 0.246-1.230 mg. The average spiked recoveries(n=6) of fructose, glucose and sucrose were 101.7%(RSD=1.6%), 102.0%(RSD=1.0%) and 100.8%(RSD=0.9%). CONCLUSION This method is accurate, rapid, simple and reproducible, and thus it is suitable for the determination of monosaccharides, disaccharides from Tanshinon extract.