Simultaneous Determination of Genistin and Genistein in Moghania Philippinens (Merr. et Rolfe) Li. from Different Places by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of genistin and genistein in Moghania philippinens (Merr. et Rolfe) Li, and compare the contents of the two components in Moghania philippinensis (Merr. et Rolfe) Li from different places. METHODS SinoChrom ODS-BP column (4.6 mm×250 mm, 5 μm) was used with acetonitrile-water solution containing 0.2% formic acid as the mobile phase in gradient elution at the flow rate of 1 mL·min-1. The temperature of column was 30 ℃, and the wavelength was 261 nm. RESULTS A good linearity was obtained over the range of 4.8-28.8 μg·mL-1 for genistin(r=0.999 8), and the average recovery(n=6) was 99.9%(RSD=1.78%), a good linearity was obtained over the range of 1.6-9.6 μg·mL-1 for genistein(r=0.999 8), and the average recovery(n=6) was 98.0%(RSD=1.17%). The contents of genistin and genistein in Moghania philippinens (Merr. et Rolfe) Li. from different places were 0.321-0.574 mg·g-1 and 0.179-0.263 mg·g-1, respectively. CONCLUSION The method used for the determination of genistin and genistein in Moghania philippinens (Merr. et Rolfe) Li. is convenient and accurate. Repeatability and stability is satisfactory. Among Moghania philippinens (Merr. et Rolfe) Li. from different places, the total contents of genistin and genistein from Yangshuo, Guangxi is the highest.