Polysaccharide Extraction from Calvatia Gigantea and its in Vitro Anti-tumor Activity

OBJECTIVE To study the optimal conditions for polysaccharide(CGP) extraction from fruiting bodies of Calvatia gigantea. METHODS First, the single factor and orthogonal tests were used to find the optimum conditions for polysaccharide extraction by hot water. Then, the proliferation inhibition effects on Cervical cancer cells (Siha) and breast cancer cells(MDA) in vitro were tested by MTT method. RESULTS Based on the single factor and orthogonal tests, the optimum extraction conditions were: extraction time 1.5 h, 2 times, temperature 100 ℃ and solid-liquid ratio 1∶15. The in vitro experiments showed that the ratio of proliferation inhibition on Siha cells reached 52.6% when the concentration of alcohol precipitation parts was 250 μg·mL^-1. Moreover, alcohol-soluble components had inhibition ratio of 80.4% for MDA cells. CONCLUSION Under above conditions, the productivity of polysaccharide extracted from fruiting bodies of Calvatia gigantean is 1.792%. And CGP has good inhibition effect on proliferations of Siha and MDA cells.