Study on Effect of Ilicifolius Alkaloids A (4-Hydroxy -2-benzoxazolone, HBOA) and Its Derivatives on α-Amylase Activity

To provide an evidence to do further study on inhibiting α-amylase and explore the application areas of Ilicifolius Alkaloids A (4-Hydroxy-2-benzoxazolone, HBOA). The effects of HBOA and its derivatives on α-amylase activity were also studied in this paper. METHODS With Acarbose as positive control, the effects of HBOA and its derivatives on α-amylase activity were compared by Bernfeld method. RESULTS a-1, a-2, a-3, a-4, a-5, b-7 could inhibit the activity of α-amylase, and the inhibition rate increased when the concentration increased in the experimental concentration range. Their inhibition rates were 10.24%, 71.52%, 63.79%, 19.71%, 52.76%, 38.96% on the 10.0 g·L^-1 concentration. While a-7, b-3, b-1 could reinforce the activity of α-amylase. CONCLUSION The author suggested that in order to better define the inhibition effects of α-amylase there is a need to synthesize some HBOA derivatives which carry chlorine atom, acyl group or hydroxyl group at position 7 of the HBOA and retain the phenolic hydroxyl group at position 4. However, taking acetoxyl group at position 4 and carboxyl group at position 7 or small group can non-yl at position 3 could reinforce the activity of α-amylase.