Study on Effect of Compound Danshen Dropping Pills on Intestinal Absorption of Aspirin in Rats

OBJECTIVE To establish a method of determination aspirin (ASP) by HPLC. Study on the effect of compound Danshen dropping pills (CDDP) on intestinal absorption in rats. METHODS The rat single-pass intestinal perfusion technique was applied to investigate the intestinal absorption of ASP, and the gravimetry was used to correct the perfusion volume. After the concentration of ASP in perfusate was determined by HPLC, the absorption parameters of ASP were calculated. RESULTS The absorption rate constants (Ka) of the ASP group, ASP combined with CDDP group and CDDP induced group were 1.05×10^-2, 1.44×10^-2 and 2.29×10^-2 min^-1, respectively. And the apparent permeability coefficients (Papp) were 0.219×10^-3, 0.304×10^-3 and 0.504×10^-3 cm·min^-1, respectively. CONCLUSION When CDDP was combined with ASP, the intestinal absorption of ASP is influenced significantly by CDDP, and CDDP could improve the intestinal absorption of ASP in rats.