Determination of Mannose in TiePiFengDou Granules by HPLC

OBJECTIVE The HPLC method for determination of Mannose in TiePiFengDou granules was established. METHODS In this method, Agilent Eclipse XDB-C₁₈ column(250 mm × 4.6 mm, 5 μm) was used. Mobile phase consisted of acetonitrile-0.02 mol·L^-1 of ammonium acetate (20∶80). Flow rate was 1.0 mL·min^-1, and detection wavelength was 250 nm. RESULTS Mannose had a good linearity within the range of 80.64-403.20 μg. The average recovery was 98.52%, RSD= 1.35%(n=6). CONCLUSION The method is accurate, convenient and reproducible. Results are stable. The method can be applied for accurately determination of mannose in TiePiFengDou granules and it is more reasonable than the determination of ginsenoside Rb1as quality standard. The method can be used as the quality control of TiePiFengDou.