Content Determination of Lysophosphatidylcholine in Seal Oil Based Lipid Emulsion for Parenteral Nutrition by HPLC-ELSD

OBJECTIVE To establish an HPLC method for determining the content of lysophosphatidylcholine in seal oil based lipid emulsion for parenteral nutrition. METHODS Using a silicone column (MZ-ANALYTICAL Spherisorb, 250 mm×4.6 mm, 5 μm) as the stationary phase, the mobile phase A was: methanol-water-glacial acetic acid-triethylamine (850∶150∶4.5∶0.5), and the mobile phase B was: methanol-water-glacial acetic acid-triethylamine-n-hexane-isopropanol (385∶68∶2∶0.23∶160∶384), and the ratio of A and B was 30∶70 with isocratic elution. The flow rate was 1.0 mL·min^-1. The injection volume was 20 μL. The column temperature was set at 40 ℃. The detector was evaporative light-scattering detector (ELSD). The drift tube temperature of the ELSD was set at 70 ℃, and the nitrogen flow rate of the ELSD was 2 L·min^-1. RESULTS The linear concentration range of lysophosphatidylcholine was 0.02-0.4 mg·mL^-1(r=0.999 4), and the average recovery was 100.3% with the RSD of 0.54%(n=9). The limit of detection was 0.12 μg(S/N=3), and the limit of quantitation was 0.40 μg(S/N=10, n=3). CONCLUSION This method is rapid, accurate, reliable and reproducible. It is suitable for the quality control of seal oil based lipid emulsion for parenteral nutrition.