Determination of Cinnamaldehyde and Piperine in Shiliu Jianwei Pill by HPLC

OBJECTIVE To determine the content of Cinnamaldehyde and Piperine in Shiliu jianwei Pill. METHODS The analysis was performed on a CAPCELL PAK C18 Column(4.6 mm×250 mm, 5 mm)at 30℃ with acetonitrile-water (40∶60)as the mobile phase and the flow rate of 1.0 mL·min^-1. The detection wavelength was 290nm. RESULITS Cinnamaldehyde have good linearity in the range of 1.149 5-13.793 5 μg(r=0.999 7). The average recovery was 97.27% and RSD was 1.39% (n=6). Piperine have good linearity in the range of 0.726 4-2.724 μg, (r=0.999 1) respectively. The average recovery was 96.51% and RSD was 1.39% (n=6). CONCLUSION The established method is accurate, reliable and specific. The quality of the pill can be controlled by the method.