Determination of Related Substances in Albendazole by HPLC

OBJECTIVE To establish an HPLC method for determination of related substances in albendazole. METHODS PAK C₁₈ HPLC packed column(MGⅡ, 250 mm×4.6 mm, 5 μm)was used, the mobile phase was ammonium dihydrogen phosphate-methanol(40∶60) and the determination wavelength was 290 nm. RESULTS Albendazole and related substances could be well isolated. The detection limit was 0.228 6 ng. CONCLUSION The method is simple, rapid, accurate and suitable for examinating the related substances of albendazole.