Determination of Total Lignanoids and Arctinin in Different Parts of Arctium Lappa L

OBJECTIVE To determine the total lignanoids and arctinin in different parts of Arctium lappa L harvested from Lintao, Gansu. METHODS According to total lignanoids and arctiin in the same peak absorption, the absorption value of the sample at 280 nm was measured to calculate content directly using arctiin as reference substance. And the content of arctiin was measured by HPLC. RESULTS The calibration curve had good linear relationship in the range of 0.005 7-0.079 5 mg·mL^-1, linear equation: Y=9.244 9X+0.017 2(r=0.999 6). The average recovery of arctiin was 98.2%, and the RSD value was 1.61%. The content of total lignanoids(in the amount of arctiin) in seed was 12.27%, and other parts of Arctium lappa L were all around 6%. The content of arctin seed was 6.19%(n=3), and the arctiin of root, leaf and stem were not found in the method. CONCLUSION The methods are simple, accurate and specific, can be used for determination of the total lignanoids and arctinin in different part of Arctium lappa L.