Determination of Contents and Related Substances of Ecabet Sodium Granules by HPLC

OBJECTIVE To establish the HPLC method for determining the contents and related substances in Ecabet sodium granules. METHODS C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-1.36% potassium dihydrogen phosphate (27︰73), and the detective wavelength was 271 nm. RESULTS The linearity was obtained over the range of 0.010 26-0.153 9 mg·mL^-1, (r=0.999 8). The average recovery was 99.8%. The related substances were well separated in the degradation test. CONCLUSION The method is simple, convenient, accurate and can be employed to assay the contents and related substances in Ecabet sodium granules for its quality control.