GC-MS Analysis of Volatile Components from Roots of Michelia Macclurei Dandy. and Growth Inhibition of NIH 3T3 Cell Line

OBJECTIVE The volatile components from roots of Michelia macclurei Dandy. were analysed, and the inhibition effects of root extract of Michelia macclurei Dandy. (REMMD) on growth of NIH 3T3 cell lines in vitro were explored. METHODS The volatile components from root of Michelia macclurei Dandy. were extracted with steam distillation method and analysed by GC-MS. The relative content of each component was calculated by peak area normalization. The inhibiting effects of REMMD on growth of NIH 3T3 cell lines in vitro were examined by MTT assay. RESULTS Fifty seven peaks were detected, in which 31 compounds were identified, which made up 63.31% of the total volatile components. CONCLUSION The main component in the volatile components is N, N’-di-benzoyloxy-heptanediamide(28.97%), other components are palmitic acid and palmitic acid ethyl ester(7.03%), 4-hydroxy-3-methoxy phenyl acetic acid (3.25%), 4-(1E)-3- hydroxy-1-propenyl-2- methoxyphenol(3.16%), costunolide(2.33%), furaldehyde and its derivatives(2.5%). REMMD with concentrations of 25, 50, 75, 100 μg·mL^?1 could significantly inhibit the growth of NIH 3T3 cell lines in vitro, therefore the results are enlightening for good researching and developing perspects of anti-fibrosis diseases.