Determination of Pinostrobin in Lindera Reflexa Helms by Near-infrared Spectroscopy Technique

OBJECTIVE To establish a rapid analytical method for pinostrobin in Lindera reflexa Helms by near-infrared(NIR) diffuse reflectance technique. METHODS Collecting NIR spectra by NIR diffuse reflectance spectroscopy with HPLC values anlysis as a reference to build calibration models by the partial least square(PLS). RESULTS The correlation and coefficients of the PLS calibration models of NIR spectroscopy for phillyrin was 0.992 31, the root-mean-square error of prediction(RMSEP) was 0.076 5, the root-mean-square error of cross-validation (RMSECV) was 0.450 0; There was no statistical difference between NIR prediction of validation samples and HPLC measurements tested by paried sample T test. CONCLUSION The method is rapid, nondestructive and accurate, and can be applied to the fast determination of pinostrobin in extractum forsythiae siccus.