Determination of Liquiritin, Glycyrrhizic Acid and Isoliquiritigenin in Xiaoyao Wan by HPLC

OBJECTIVE To develop an HPLC method for the liquiritin, glycyrrhizic acid and isoliquiritigenin in Xiaoyao Wan. METHODS The column Shim-pack VP-ODS(150 mm×4.6 mm，5.0 μm)was used. The mobile phase consisted of methanol-0.5% acetic acid with a gradient elution, and the flow rate was 1.0 mL·min^-1. The detection wavelengthes were 276 nm in 0-7 min, 370 nm in 7-18 min, 254 nm after 18 min． RESULTS The linear range of liquiritin was 0.018 40-0.368 0 μg, r=0.999 8. The average recovery was 97.96%, RSD=0.60%. The linear range of glycyrrhizic acid was 0.015 20-0.304 0 μg , r=0.999 9. The average recovery was 97.17%, RSD=1.02%. The linear range of isoliquiritigenin was 0.441 8-8.835 0 μg , r=0.999 9. The average recovery was 97.52%, RSD=1.06%. CONCLUSION This method is simple, feasible, and the repeatability is good. It can be used for quality control of Xiaoyao Wan.