Determination of Epigoitrin Content in Ganmao Tuire Granules by HPLC

OBJECTIVE To establish a method for rapid determination of epigoitrin content in Ganmao Tuire Granules. METHODS The sample was extracted by water and separated by Inertsil ODS-SP column, the mobile phase was acetonitrile-triethylamine phosphoric acid solution (included 0.08% phosphoric acid and 0.04% triethylamine) (5∶95), the detection wavelength was at 245 nm , flow rate was 1.0 mL·min^-1, the column temperature was at 30 ℃. RESULTS The linear range of epigoitrin was in 0.010 44-0.522 μg(r=0.999 9). The average recovery was 99.7% with RSD of 0.79% (n=9). The epigoitrin content in Ganmao Tuire Granules was obviously different from different pharmaceutical factories. CONCLUSION This method is simple, rapid and reproducible, and it can be used as a low-cost, convenient method for measuring epigoitrin content in Ganmao Tuire Granules, and it can provide reference for relative company and state food and drug administration.