Determination of Hesperdin in Expectorant Mixture by HPLC

OBJECTIVE An HPLC method was developed and validated for determining hesperdin in Expectorant mixture. METHODS Chromatographic separation was performed on an Agilent Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm) with a mobile phase of methanol-glacial acetic acid-distilled water(25∶5∶70). The flow rate was 1.0 mL·min^-1 and the detecting wavelength was 283 nm. RESULTS The linear range of hesperdin was 1.05-34.56 μg·mL^-1. Linear equation was Y=10.346X+0.795 2(r=0.999 6). The average recovery (n=9) of hesperdin was 98.3% (RSD=1.7%). CONCLUSION The method is simple, accurate and selective and sensitive to quantify hesperdin in Expectorant mixture.