Determination of Cefprozil by HPLC in Human Plasma and Its Pharmacokinetics

OBJECTIVE To develop a high performance liquid chromatography method for the determination of cefprozil in human plasma and to study its pharmacokinetics. METHODS The human plasma samples were precipitated by perchloric acid. The analytical column was ZORBAX Eclipse XDB-C₈. The mobile phase was acetonitrile-0.1% trifluoroacetic acid-water(15∶40∶45) and the flow rate was 1.0 mL·min^-1. The UV detection wavelength was 282 nm. RESULTS Excellent liner relationship was obtained in the range of 0.10-20.00 mg·L^-1(r=0.999 9). The relative recoveries were (99.20±4.41)%, (98.07±3.94)%, and (100.07±1.69)% respectively at three concentrations. The intra-day RSD were 5.28%, 5.19%, and 1.97% and inter-day RSD were 4.38%, 3.95%, and 1.33% respectively. Cefprozil was fit to the one-compartment model with first absorption, C_max was (8.79±1.09)mg·L^-1, t_1/2(ke) was (1.06±0.29) h. CONCLUSION The method was simple, rapid, and accurate. It can be used to determine the cefprozil concentration in human plasma and to study of its pharmacokinetics.