Chemometric Analysis on the GC Fingerprint Data of the Root of Bupleurum chinense DC

OBJECTIVE To study the gas chromatographic fingerprint of the volatile constituents from the root of Bupleurum chinense DC. and obtain the characteristic data based on chemometric analysis. METHODS Samples of B.chinense DC. from different habitats were determined by Aglient 7890A GC system with HP-5 capillary column (30 m×0.25 mm, 0.25 µm). Nitrogen was used as carrier gas and flow rate was 1.03 mL·min-1, FID temperature was 280 ℃. The methods of similarity, cluster analysis and principal component analysis were used to process the data from which was obtained gas chromatographic fingerprint. The assisted computational tool was “Chromafinger” solution software. RESULTS The common pattern of GC fingerprint was established. Twenty-eight co-possessing peaks were selected as the fingerprint peaks of B.chinense DC.. Samples were divided into three categories based on the analysis of chemometrics. CONCLUSION The results show that chemometrics is suitable for the analysis of fingerprint data, which can provide a basis for quantitative research. Chemometric analysis contributes to research the quality evaluation for B. chinense DC..