Resonance Rayleigh Scattering Spectra of Interaction of Amoxicillin with Methyl Violet and Their Analytical Applications

OBJECTIVE To develop a new sensitive Resonance Rayleigh scattering(RRS) method for the determination of trace amoxicillin. METHODS RRS spectra method was used for the determination of amoxicillin with methyl violet as probe. RESULTS In pH 9.90 Tris-HCl buffer solution, the RRS intensity of methyl violet was greatly enhanced when amoxicillin was added. And the strongest ΔIRRS was located at 376 nm. The intensity of RRS was in proportion to the concentration of amoxicillin in the range of 2.0×10^-8-1.5×10^-6 mol·L^-1 with a detection limit(3Sb/S) of 0.042 µg·mL^-1. CONCLUSION The method showed advantage of convenience, rapidity, high sensitivity and was applied in the determination of trace amoxicillin with suitable results.