Synthesis and Activity of Rhein Lysinate

OBJECTIVE To prepare the derivate of Rhein dissoluble in water. METHODS The target compound was prepared from Rhein, reacted at 30 ℃ for 24 h. Then it was precipitated and dried. The determination of the dissolubility of Rhein lysinate was based on Ch.P(2005). The effect of Rhein lysinate on cell proliferation was detected by MTT. RESULTS The structure of new compound was confirmed by HPLC, IR and 1H-NMR. The dissolubility of Rhein lysinate in water was 15 g·L^-1. CONCLUSION The derivate of Rhein, Rhein lysinate, can dissolve in water and have the same effect on cell proliferation with Rhein.