Content Determination of Astragaloside in Compound Shenqi Soft Capsule by HPLC-ELSD

OBJECTIVE To establish an HPLC-ELSD method for the determination of astragaloside in compound shenqi soft capsule. METHODS The samples were determined by HPLC-ELSD on an Agilent Hypersil ODS column using acetonitrile-water(35∶65) as the mobile phase at a flow rate of 1.0 mL·min^-1. The injection volume was 20 μL. The ELSD conditions were as follows: pressure of compressed air was 0.32 MPa, the flow rate of compressed air was 2.6 L·min^-1，drift temperature was 108 ℃. RESULTS The linear range of astragaloside was 0.402 4-4.024 0 µg (r=0.999 8) and the average recovery rate was 100.02%(RSD=1.41%). CONCLUSION The method is accurate, specific and applicable for the determination of astragaloside in compound shenqi soft capsule.