Determination of Astragaloside Ⅳ in Aikefu Granules by HPLC-ELSD

OBJECTIVE To establish a method for the determination of astragaloside Ⅳ in Aikefu granules by HPLC-ELSD. METHODS The content of astragaloside Ⅳ was determined by HPLC-ELSD. RESULTS A good linearity was observed for astragaloside Ⅳ in the range of 0.64-20.48 μg. The average recovery of astragaloside Ⅳ was 97.78% with RSD=1.12%(n=6). CONCLUSION The method is simple, accurate and reiable. It can be used for the quality control of granules.