Determination of Gallic Acid in Leaf of Liquidambar taiwaniana Hance in Different Areas by HPLC

OBJECTIVE To establish the method of determination of gallic acid in Leaf of Liquidambar taiwaniana Hance in different areas. METHODS The chromatographic conditions included Alltima C₁₈ column(4.6 mm×250 mm, 5 μm) and the mobile phase consisted of acetonitril-0.05% phosphoric acid(4∶96). Detection wavelength was at 270 nm. The flow rate was 1.0 mL·min^-1. Column temperature was at 30 ℃. RESULTS There was a good linear within the range of 0.019 08-0.229 0 mg·mL^-1 for Gallic acid with r=0.999 6. The average recovery was 101.52% with RSD=1.40%(n=6). CONCLUDION The method is simple, accurate, reproducible and can be used for the quality control of Liquidambar taiwaniana Hance.