Preparation and Characteristics of Daidzein Solid Lipid Nanoparticles

OBJECTIVE To prepare and investigate daidzein solid lipid nanoparticles. METHODS The formulation of daidzein solid lipid nanoparticles were optimized by orthogonal experiments. The daidzein solid lipid nanoparticles was investigated based on the experiments of its particle size, ζ potential, entrapment efficiency, stability and cumulated released rate. RESULTS The most optimal formulation was as follows: content of mono-stearate was 2.0%, content of daidzein was 2.0 mg·mL^-1, content of soybean lecithin was 0.4%, content of Pluronic F68 was 1.2%. Entrapment efficiency of new daidzein solid lipid nanoparticles was 84.7% , ζ potential was -35.8 mV, particle size was 170 nm, cumulated released rate within 72 hours was 90.3%. CONCLUSION A new stable daidzein solid lipid nanoparticles is attainable.