Preparation and Characterization of Isoniazid Gelatin Microspheres for Passive Lung Targeting

OBJECTIVE Lung targeting gelatin microspheres of isoniazid (INH-GMS) were prepared using the biodegradable materials-gelatin. METHODS INH-GMS were prepared using the emulsifying method and the effect of some factors on the technology was analyzed with the orthogonal test design. The morphology of the microspheres was observed by upright fluorescence microscope. The formation of INH-GMS was confirmed by differential scanning calorimetry (DSC) and infrared spectrometry (IR). The mean diameter and the size distribution, drug-loading rate, entrapment efficiency and stability of INH-GMS were examined. RESULTS INH was developed in microspheres and INH-GMS were regular in morphology. The mean diameter of INH-GMS was 15.63 μm, more than 90% of the INH-GMS ranging from 5.0 μm to 25.0 μm. The drug-loading rate and entrapment efficiency were 14.93% and 73.30% respectively. The repeatability of the technology was good. The content, shape and size of the INH-GMS showed no remarkable change after storage at room temperature for 6 months. CONCLUSION The technology of preparation was successful and the INH-GMS can be used for study of passive lung target.