Determination of Chlorogenic Acid and Caffeic Acid in Chaocangerzi Dispensing Granule by HPLC

OBJECTIVE To develop an HPLC method for simultaneous determination of chlorogenic acid and caffeic acid in Chaocangerzi dispensing granule. METHODS Chlorogenic acid and caffeic acid were separated on Shim-pack VP-ODS (250 mm×4.6 mm, 5 μm) column and detected at 327 nm, 35 ℃, with acetonitrile-0.1% phosphoric acid solution (10∶90) as mobile phase. RESULTS The method had good linear relationship within the range of 0.215 2-1.721 6 μg (r=0.999 9) for chlorogenic acid and 0.022 96-0.114 8 μg (r=0.999 9) for caffeic acid, respectively. The results of the average recovery were 96.39% (RSD=1.48%) and 100.02% (RSD=1.81%). CONCLUSION The method is simple, accurate and can be used to simultaneously determine chlorogenic acid and caffeic acid in Chaocangerzi dispensing granule.