Determination of the Ginkgo Flavonoid Aglycon Solid Dispersions Capsules by HPLC

OBJECTIVE A HPLC method was established to determine the content of ginkgo flavonoid aglycon solid dispersions capsules. METHODS Chromatographic condition included the Hypersil ODS column (4.6 mm×150 mm, 5 μm) and mobile phase consisting of methanol - 4‰ acetic acid solution(48∶52). The flow rate was 0.8 mL·min^-1, the detection wavelength was set at 360 nm, the column temperature was 45 ℃. RESULTS The linear calibration curves were obtained in the concentration range of 5.0-100.0 μg·mL^-1 and 3.40-68.0 μg·mL^-1. The mean recovery was 99.31%, 99.35% and 98.18%, RSD was 2.12%, 2.28% and 1.65%(n=6) with good precision. CONCLUSION The method is sensitive, accurate and high selectivity. A satisfactory result to determine the dissolution of this dosage form was obtained with this method.