Study on Composition and Immunomodulating Activities of Extracellular Polysaccharide from Culture Medium of Phellinus pini

OBJECTIVE Study on monosaccharide composition and immunomodulating activities of extracellular polysaccharide of Phellinus pini. METHODS PPE was purified from CPPE using a combination of techniques, such as: frozen-thawed, deproteinated by enzyme/Sevag method and Sepharose CL-6B column chromatography. Homogenicity and molecular weight of PPE were analyzed by HPLC, and its composition was assayed by GC. RESULTS PPE appeared as single symmetrical peak on HPLC, and the average molecular weights was 39 kDa. Monosaccharide analysis by GC shows that it was mannan. The bioassay showed that PPE could stimulate mouse splenic lymphocytes proliferation and increase the ConA or LPS-induced lymphocytes proliferation in vitro. CONCLUSION The results showed that PPE have the function of enhancing the immunity.