Naphthalenyl Glucosides from the Cytotoxic Portion of the Fresh Rejuvenated Fruits of Juglans Mandshurica

OBJECTIVE To study the chemical components of the cytotoxic portion of the fresh rejuvenated fruits of Juglans mandshurica. METHODS The cytotoxicity of four elutes from macro-porous resin chromatography was estimated by MTT assay against human stomach BGC803 cancer cells. The 30% ethanol elute showing the strongest activities was isolated by silica gel chromatography and reverse phase silica gel chromatography. RESULTS 1,4,5-trihydroxynaphthalene-1,4- di-O-β-D-glucopyranoside(1), 1,4,5-trihydroxynaphthalene-1,5-di-O-β-D-glucopyranoside(2), 1,4,8-trihydroxynaphthalene-1-O-β-D-glucopyranoside(3), 1,4,8-trihydroxynaphthalene-1-O-β-D-6′-O-(3″,4″,5″-trihydroxybenzoyl) glucopyranoside(4) were isolated and their structures were determined by NMR spectrum. CONCLUSION There are naphthalenyl glucosides in the cytotoxic portion of fresh rejuvenated fruits of Juglans mandshurica and they may play a significant role in its antitumor activities.