Protective Effects of Adiponectin on Oxidative Damage of EPCs and Its Possible Mechanism

OBJECTIVE To observe the protective effects of adiponectin on oxidative low density lipoprotein(Ox-LDL)- induced oxidative damage of endothelial progenitor cells (EPCs) from peripheral blood. And study its possible mechanism. METHODS Total mononuclear cells(MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and were identified by demonstrating the expression of CD34, VEGFR-2 and AC133 with flow cytometry, to make sure that all the cells needed were EPCs. Then the cells were plated on fibronectin-coated culture dishes. After incubation for 7 days, attached cells were collected and divided into three groups: control group, Ox-LDL group(10 µg·mL^-1), adiponectin intervention group, stimulated with different cencentrations of adiponectin (1.25, 2.5, 5 µg·mL^-1). The cells viability was examined by methyl thiazoiyl tetrazolium(MTT) assay after intervented for 24 h. And cell supernate of each group was carried on the SOD, MDA content examination. RESULTS In Ox-LDL group, the cells viability and SOD content obviously droped, the MDA content obviously elevated. The cells viability and SOD content of adiponectin intervention group obviously increased and the MDA content obviously reduced. CONCLUSION Adiponectin has protective effects on the oxidative injured EPCs induced by Ox-LDL. Its mechanism may be related to the function of anti-oxidase activity.